Lysozyme 0.150 g 210 mL osmotic pressure 0.127 kPa at 25℃

Lysozyme 0.150 g 210 mL osmotic pressure 0.127 kPa at 25℃

 

 

Lysozyme is an enzyme that breaks bacterial cell walls. A solution containing 0.150 g of this enzyme in 210 mL of solution has an osmotic pressure of 0.127 kPa at 25℃. What is the molar mass of lysozyme?

 

 

 

1 atm = 101.325 kPa 이므로,

0.127 kPa × (1 atm / 101.325 kPa)

= 0.127 × (1 / 101.325)

= 0.00125339 atm

 

 

 

π = MRT

( 참고 https://ywpop.tistory.com/1921 )

 

 

 

M = π / RT

= 0.00125339 / (0.08206 × (273.15 + 25))

= 5.123×10^(-5) mol/L

 

 

 

(5.123×10^(-5) mol/L) (210/1000 L)

= 1.0758×10^(-5) mol

( 참고 https://ywpop.tistory.com/7787 )

 

 

 

0.150 g / (1.0758×10^(-5) mol)

= 13943 g/mol

( 참고 https://ywpop.tistory.com/7738 )

 

 

 

답: 1.39×10^4 g/mol

 

 

 

 

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