An enzyme (M_r 30 000) is available commercially

An enzyme (M_r 30 000) is available commercially

 

 

An enzyme (M_r 30 000) is available commercially as 0.2 g/100 mL at 80% purity.

What volume (in whole mL) would you take to have around 0.1 μmol enzyme needed for an assay?

 

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몰수를 질량으로 환산하면,

(0.1*10^-6 mol) * (30000 g/mol) = 0.003 g

( 계산 설명 http://ywpop.tistory.com/7738 )

 

 

0.003 g을 포함하는 시약의 부피를 계산하면,

0.003 g / (0.2 g/100 mL) = 1.5 mL

 

 

80% 순도를 고려하면,

1.5 mL / (80/100) = 1.875 mL

 

 

답: 1.9 mL