반응형 buffer2 How to Prepare 1 M Tris Buffer pH 8.0 How to Prepare 1 M Tris Buffer pH 8.0 You want to prepare 1 M Tris buffer pH 8.0. ▶ Tris = tris(hydroxymethyl)aminomethane 또는 2-Amino-2-(hydroxymethyl)propane-1,3-diol After dissolving 121.1 g of Tris base in 800 mL of water, you added x mL of HCl and adjusted the final volume to 1 liter. How much HCl did you add? pKa value for Tris is 8.1. MW of Tris = 121.14 g/mol. 10^0.1 = 1.26. density of HC.. 2014. 10. 27. N2H4-N2H5Cl 완충 용액 (buffer system) N2H4-N2H5Cl 완충 용액 (buffer system) --------------------------------------------------- ▶ 참고: NH3-NH4Cl 완충 용액의 완충 작용 [ https://ywpop.tistory.com/5442 ] --------------------------------------------------- N2H4 / N2H5^+ buffer system은 N2H4가 weak base이기 때문에, basic buffer에 해당된다. ---> NH3 / NH4^+와 같은 약염기 완충 용액 따라서 산 또는 염기를 첨가했을 때 일어나는 반응 또한 동일하다. NH3(aq) + HCl(aq) → NH4^+(aq) + Cl^-(aq) NH4Cl(aq) + NaO.. 2014. 10. 20. 이전 1 다음 반응형
